Journal: Redox Biology

Article Title: Binding of the human antioxidation protein α 1 -microglobulin (A1M) to heparin and heparan sulfate. Mapping of binding site, molecular and functional characterization, and co-localization in vivo and in vitro

doi: 10.1016/j.redox.2021.101892

Figure Lengend Snippet: Immunofluorescence staining of organs from 10-week-old female C57BL/6NRj mice. Lung, aorta, kidney and skin stained with Alexa Fluor 488-anti-heparan sulfate (green), Alexa Fluor 647-anti A1M (red) and Hoechst (blue). Images were obtained on a Widefield Epifluorescence Ti2 microscope equipped with a Nikon DS-Qi2 camera. Magnification 10x (left column) and 40x (right column). Scale bars, 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Microscopy was performed on a Widefield Epifluorescence Ti2 microscope equipped with a Nikon DS-Qi2 camera (Nikon, Minato City, Tokyo, Japan).

Techniques: Immunofluorescence, Staining, Microscopy